Starch Branching Degree Determination

Starch in nature can be roughly divided into two categories: amylose and amylopectin. Amylopectin has different numbers of branches (i.e., 1,6-glucosidic bond forms), and this indicator is an important indicator of the degree of branching of amylopectin. The higher the degree of branching per unit chain length, the greater the number of branches. Since starch branches form 1,6-glycosidic bonds, which are different from the main chain 1,4-glycosidic bonds, they can be distinguished based on this characteristic. The nuclear magnetic method was used to determine the number of 1,4-glycosidic bonds and 1,6-glycosidic bonds in starch to determine the branching degree of starch. This method is stable and can accurately reflect the branching degree of starch.

 

Workflow

 

Why Choose GlycoSpectra Analytics

1.Good stability, RSD<5% of samples from the same batch.

 

2.Manually assisted subsequent analysis to provide well-analyzed data.

 

3.Thorough service, providing pre-sale consultation and post-sale technical support.

 

Sample Submission Guidelines

1.Sample Types

 

Starch samples, clients can provide the original sample for starch extraction by our company, which is subject to a separate charge.

 

2.Biological Replicates

 

A minimum of three biological replicates is recommended for robust results.

 

3.Quantity Requirements

 

Raw sample > 50 g; Starch >1 g.

 

4.Sample Preparation

 

1)Establish both control and experimental groups, ensuring a minimum of three biological replicates.

2)Ensure maintain consistency in timing whenever possible.

3)Minimize preprocessing; we prefer to handle these steps. Clients should ensure liquid samples are securely packaged in non-glass containers and buffered against shock and impact. Dry solid samples can be untreated or preliminarily ground. Fresh solid samples require no treatment when shipped with ice packs at ambient temperature.

4)When shipping with dry ice, samples should be cut into small pieces, each less than 1 cm³.

 

5.Packing and Shipping

 

1)Store samples in clearly labeled tubes, label with sample name, concentration, and preparation date. Ensure tube tops are sealed.

2)For transport, secure sample tubes in 50ml capped centrifuge tubes placed in sealable bags. Use dry ice or ice packs for transportation, avoiding repeated freeze-thaw cycles. DO NOT send the samples out on Fridays, to avoid weekend delays.

 

6.Labeling Requirements

 

1)Employ double labeling, ensuring clear identification on both the sample tubes and external packaging.

2)To prevent confusion, avoid simplistic labels like A/B/C or 1/2/3. Instead, use a combination of letters and numbers for unique sample identification, reducing the risk of duplication.

 

7.Additional Information

 

A minimum of 24 analyses is required. If fewer than 24 analyses are conducted, the cost will be calculated as if 24 analyses were performed.