Measurement of Resistant Starch Content

Resistant starch(RS), also known as enzyme-resistant starch and indigestible starch, can not be hydrolyzed by enzymes in the small intestine, but can ferment with volatile fatty acids in the human gastrointestinal tract and colon. According to their properties, they are generally divided into four categories: RSI, RS2, RS3 and RS4. Non-resistant starch was removed by α-pancreatic amylase and starch glucosidase, residue was dissolved by KOH, acetic acid buffer was adjusted to neutral, and starch was hydrolyzed to glucose by starch glucosidase, Glucose Oxidase-Peroxidase (GOPOD) reagent buffer reacted with D-Glucose to form imine dye, and its content was determined by colorimetry.

 

Workflow

 

Why Choose GlycoSpectra Analytics

1.High throughput, 96 samples can be ordered at one time.

 

2.Humanized solution, either full powder or amyloid.

 

3.Convenient and fast, capable of delivering results within 20 working days.

 

4.Thorough service, providing pre-sale consultation and post-sale technical support.

 

Sample Submission Guidelines

1. SampleTypes

 

Fresh or dry samples are both acceptable.

 

2.Biological Replicates

 

A minimum of three biological replicates is recommended for robust results.

 

3.Quantity Requirements

 

Fresh sample > 2 g; Dry sample >1 g.

 

4.Sample Preparation

 

1)Establish both control and experimental groups, ensuring a minimum of three biological replicates.

2)Ensure maintain consistency in timing whenever possible.

3)Minimize preprocessing; we prefer to handle these steps. Clients should ensure liquid samples are securely packaged in non-glass containers and buffered against shock and impact. Dry solid samples can be untreated or preliminarily ground. Fresh solid samples require no treatment when shipped with ice packs at ambient temperature.

4)When shipping with dry ice, samples should be cut into small pieces, each less than 1 cm³.

 

5.Packing and Shipping

 

1)Store samples in clearly labeled tubes, label with sample name, concentration, and preparation date. Ensure tube tops are sealed.

2)For transport, secure sample tubes in 50ml capped centrifuge tubes placed in sealable bags. Use dry ice or ice packs for transportation, avoiding repeated freeze-thaw cycles. DO NOT send the samples out on Fridays, to avoid weekend delays.

 

6.Labeling Requirements

 

1)Employ double labeling, ensuring clear identification on both the sample tubes and external packaging.

2)To prevent confusion, avoid simplistic labels like A/B/C or 1/2/3. Instead, use a combination of letters and numbers for unique sample identification, reducing the risk of duplication.

 

7.Additional Information

 

A minimum of 24 analyses is required. If fewer than 24 analyses are conducted, the cost will be calculated as if 24 analyses were performed.