Determination of Starch Molecular Weight Distribution
Molecular weight distribution is the core parameter of starch fine structure. Starch is a polymer formed by the polymerization of glucose, which has very poor solubility due to the extremely high degree of polymerization.At present, salt phase heating or alkali solution heating is used to dissolve starch, and then gel chromatography is used to separate the column, combine the differential detector and dextran standard to determine the molecular weight distribution of starch, this method cannot fully dissolve the starch, resulting in a large deviation in the results. GlycoSpectra Analytics uses gel exclusion chromatography combined with a differential index detector and a laser light scattering detector, and uses Dimethyl sulfoxide(DMSO) as the dissolving solution and mobile phase to maximize the dissolution of starch to obtain the most accurate and realistic starch molecular weight data.
Workflow
Why Choose GlycoSpectra Analytics
1.Dual detectors for differential index and laser light scattering for more reliable absolute quantitation results.
2.DMSO system, starch dissolution is more complete, and the determination is more accurate.
3.Multiple columns are connected in series, with a wide detection range and molecular weight range of 103-107.
4.Rich experience and completion of numerous projects.
Sample Submission Guidelines
1.Sample Types
Starch samples, clients can provide the original sample for starch extraction by our company, which is subject to a separate charge.
2.Biological Replicates
A minimum of three biological replicates is recommended for robust results.
3.Quantity Requirements
Starch >1 g; Raw sample > 50 g.
4.Sample Preparation
1)Establish both control and experimental groups, ensuring a minimum of three biological replicates.
2)Ensure maintain consistency in timing whenever possible.
3)Minimize preprocessing; we prefer to handle these steps. Clients should ensure liquid samples are securely packaged in non-glass containers and buffered against shock and impact. Dry solid samples can be untreated or preliminarily ground. Fresh solid samples require no treatment when shipped with ice packs at ambient temperature.
4)When shipping with dry ice, samples should be cut into small pieces, each less than 1 cm³.
5.Packing and Shipping
1)Store samples in clearly labeled tubes, label with sample name, concentration, and preparation date. Ensure tube tops are sealed.
2)For transport, secure sample tubes in 50ml capped centrifuge tubes placed in sealable bags. Use dry ice or ice packs for transportation, avoiding repeated freeze-thaw cycles. DO NOT send the samples out on Fridays, to avoid weekend delays.
6.Labeling Requirements
1)Employ double labeling, ensuring clear identification on both the sample tubes and external packaging.
2)To prevent confusion, avoid simplistic labels like A/B/C or 1/2/3. Instead, use a combination of letters and numbers for unique sample identification, reducing the risk of duplication.
7.Additional Information
A minimum of 24 analyses is required. If fewer than 24 analyses are conducted, the cost will be calculated as if 24 analyses were performed.