Amylase Activity Analysis Service
Enzymes are biomolecules with high molecular weight, known for their biological catalytic functions. In enzymatic catalytic systems, the reactant molecules are known as substrates, which are transformed into different molecules through the catalysis of enzymes. As key facilitators of numerous biological processes, enzyme activity levels are indicative of an organism’s internal state. The detection of certain enzyme activities becomes feasible due to the characteristic absorbance at specific wavelengths of some substrates involved in the catalytic reactions of biological enzymes. The synthesis and metabolism of starch involve a series of enzyme-catalyzed reactions. Key enzymes in this process include the synthesis enzyme system: Adenosine Diphosphoglucose Pyrophosphprylase (AGP), Granule-Bound Starch Synthase(GBSS), Starch Branching Enzyme(SBE), Soluble Starch Synthase(SSS), etc.; and the degradation enzyme system: α-Amylase, β-Amylase, Acid sucrose invertase(AI), Neutral sucrose invertase(NI), Sucrose phosphate synthase(SPS), etc. GlycoSpectra Analytic has developed an enzymatic activity assay system for the starch metabolism pathway, enabling the rapid, efficient, and accurate determination of various enzymatic activity indicators.
List of Service
- Adenosine diphosphoglucose pyrophosphprylase (AGP)
- Granule-bound starch synthase (GBSS)
- Starch branching enzyme (SBE)
- Soluble starch synthase (SSS)
- α-Amylase
- β-Amylase
- Acid sucrose invertase (AI)
- Neutral sucrose invertase (NI)
- Sucrose phosphate synthase (SPS)
Workflow
Why Choose GlycoSpectra Analytics
1.High-throughput capability, allowing for the simultaneous processing of 96 samples in a single run.
2.Excellent stability, with a Relative Standard Deviation (RSD) of less than 5% within the same batch of samples.
3.Our process is streamlined and efficient, delivering results within 20 working days.
4.We offer a full spectrum of services, encompassing pre-sale consultations and post-sale technical support.
Sample Submission Guidelines
1.Sample Types
Plant tissues such as roots, stems, leaves, etc..
2.Biological Replicates
A minimum of three biological replicates is recommended for robust results.
3.Quantity Requirements
Fresh sample > 1 g; Liquid nitrogen sampling, dry ice mailing;
4.Sample Preparation
1)Establish both control and experimental groups, ensuring a minimum of three biological replicates.
2)Ensure maintain consistency in timing whenever possible.
3)Minimize preprocessing; we prefer to handle these steps. Clients should ensure liquid samples are securely packaged in non-glass containers and buffered against shock and impact. Dry solid samples can be untreated or preliminarily ground. Fresh solid samples require no treatment when shipped with ice packs at ambient temperature.
4)When shipping with dry ice, samples should be cut into small pieces, each less than 1 cm³.
5.Packing and Shipping
1)Store samples in clearly labeled tubes, label with sample name, concentration, and preparation date. Ensure tube tops are sealed.
2)For transport, secure sample tubes in 50ml capped centrifuge tubes placed in sealable bags. Use dry ice or ice packs for transportation, avoiding repeated freeze-thaw cycles. DO NOT send the samples out on Fridays, to avoid weekend delays.
6.Labeling Requirements
1)Employ double labeling, ensuring clear identification on both the sample tubes and external packaging.
2)To prevent confusion, avoid simplistic labels like A/B/C or 1/2/3. Instead, use a combination of letters and numbers for unique sample identification, reducing the risk of duplication.
7.Additional Information
A minimum of 24 analyses is required. If fewer than 24 analyses are conducted, the cost will be calculated as if 24 analyses were performed.