Polysaccharide Extraction and Separation
Polysaccharide is a long-chain polymer composed of multiple monosaccharide molecules linked to each other by glycosidic bonds, and is a very important macromolecular substance in cells. Carbohydrates are important components of receptor molecules on cell membranes, participants in cell recognition and information transmission functions, and are a class of non-specific broad-spectrum immunomodulators and important vital material materials, which are widely involved in various life activities. Studies in recent years have shown that polysaccharides have shown good application prospects in immunomodulation, antitumor, antiviral, antioxidant and hypoglycemic aspects. Polysaccharides are the common focus of modern medicine and food functional chemistry.
Natural polysaccharides come from a wide range of sources and have complex chemical compositions, and crude polysaccharides are usually accompanied by some proteins, fats, pigments and other substances, which are not conducive to the structural identification and subsequent activity analysis of polysaccharides, so separation and purification are required: that is, impurity removal and component separation. After years of technology accumulation, GlycoSpectra Analytics has established a complete polysaccharide extraction and purification platform. The process begins with grinding the sample into powder, followed by hot water extraction, alcohol precipitation, protein removal, decolorization, and defatting, resulting in crude polysaccharides. These crude polysaccharides are then purified through ion exchange chromatography and gel chromatography to obtain corresponding single, uniform polysaccharide components.
Workflow
Why Choose GlycoSpectra Analytics
1.Rich separation and purification experience to ensure high yield and high purit.
2.Professional and advanced equipment, utilizing the U.S.-made GE AKTA explorer automatic large molecule purification system, compatible with various purification chromatography columns, offering a wide range of separation capabilities.
3.Comprehensive detection services, providing multiple testing indicators for polysaccharides (such as chemical composition, purity, etc.), meeting various scientific research needs.
4.Convenient and efficient, supporting personalized customization of polysaccharide extraction, impurity removal, and purification research plans.
Sample Submission Guidelines
1.Sample Types
Plants (medicinal materials, vegetables, fruits, etc.), fungi, marine organisms, etc.
2.Quantity Requirements
The dry weight of the raw material is recommended to be ≥1kg.
3.Sample Preparation
1)Fresh samples are recommended to be dried and crushed first to avoid decay during shipping.
2)Sampling should be uniform, and the sampling location should be consistent to ensure sample homogeneity.
3)f the raw material is sufficient, it is advisable to increase the quantity of samples sent.
4.Packing and Shipping
Please place the sample in a suitable container or sample bag, ensuring it is well-sealed to prevent leakage during transportation.
5.Labeling Requirements
1)Employ double labeling, ensuring clear identification on both the sample tubes and external packaging.
2)To prevent confusion, avoid simplistic labels like A/B/C or 1/2/3. Instead, use a combination of letters and numbers for unique sample identification, reducing the risk of duplication.
6.Other
A sample technical requirement form must be mailed along with the samples to avoid delays in project timelines due to the inability to promptly verify information.