Starch Phosphorylation Assay

Starch is a polymer composed of single glucose molecules, where the hydroxyl groups at the 3rd and 6th positions of the glucose molecules can be substituted by phosphate groups, thereby forming phosphorylated glucose.In the early stages of starch synthesis or degradation, glucose molecules first undergo a phosphorylation reaction, before participating in the corresponding enzyme-catalyzed reaction systems. The degree of phosphorylation modification of starch to some extent reflects the digestibility characteristics of starch.

 

Workflow

 

Why Choose GlycoSpectra Analytics

1.Rich experience and completion of numerous projects.

 

2.Artificial assistance in analysis, providing processed data.

 

3.Comprehensive service, offering pre-sale consultations and after-sales technical support.

 

Sample Submission Guidelines

1.Sample Types

 

Starch samples, clients can provide the original sample for starch extraction by our company, which is subject to a separate charge.

 

2.Biological Replicates

 

A minimum of three biological replicates is recommended for robust results.

 

3.Quantity Requirements

 

Raw sample > 50 g; Starch >1 g.

 

4.Sample Preparation

 

1)Establish both control and experimental groups, ensuring a minimum of three biological replicates.

2)Ensure maintain consistency in timing whenever possible.

3)Minimize preprocessing; we prefer to handle these steps. Clients should ensure liquid samples are securely packaged in non-glass containers and buffered against shock and impact. Dry solid samples can be untreated or preliminarily ground. Fresh solid samples require no treatment when shipped with ice packs at ambient temperature.

4)When shipping with dry ice, samples should be cut into small pieces, each less than 1 cm³.

 

5.Packing and Shipping

 

1)Store samples in clearly labeled tubes, label with sample name, concentration, and preparation date. Ensure tube tops are sealed.

2)For transport, secure sample tubes in 50ml capped centrifuge tubes placed in sealable bags. Use dry ice or ice packs for transportation, avoiding repeated freeze-thaw cycles. DO NOT send the samples out on Fridays, to avoid weekend delays.

 

6.Labeling Requirements

 

1)Employ double labeling, ensuring clear identification on both the sample tubes and external packaging.

2)To prevent confusion, avoid simplistic labels like A/B/C or 1/2/3. Instead, use a combination of letters and numbers for unique sample identification, reducing the risk of duplication.

 

7.Additional Information

 

A minimum of 24 analyses is required. If fewer than 24 analyses are conducted, the cost will be calculated as if 24 analyses were performed.