Starch Analysis by Infrared Spectroscopy

The near-infrared spectrum is mainly generated when the non-resonance of molecular vibration causes the molecular vibration to transition from the ground state to a high energy level. What is recorded is mainly the frequency doubling and combined frequency absorption of the vibration of the hydrogen-containing group X-H (X=C, N, O), very suitable for measuring the composition and properties of hydrocarbon organic substances. The Fourier transform infrared spectrometer(FT-IR) is used to analyze the infrared absorption spectrum of the sample to be tested, which can effectively reflect the ordered structure of starch granules.

 

Workflow

 

Why Choose GlycoSpectra Analytics

1.Good stability, RSD<5% of samples from the same batch.

 

2.Rich experience and completion of numerous projects.

 

3.Thorough service, providing pre-sale consultation and post-sale technical support.

 

Sample Submission Guidelines

1.Sample Types

 

Starch samples, clients can provide the original sample for starch extraction by our company, which is subject to a separate charge.

 

2.Biological Replicates

 

A minimum of three biological replicates is recommended for robust results.

 

3.Quantity Requirements

 

Raw sample > 50 g; Starch >1 g.

 

4.Sample Preparation

 

1)Establish both control and experimental groups, ensuring a minimum of three biological replicates.

2)Ensure maintain consistency in timing whenever possible.

3)Minimize preprocessing; we prefer to handle these steps. Clients should ensure liquid samples are securely packaged in non-glass containers and buffered against shock and impact. Dry solid samples can be untreated or preliminarily ground. Fresh solid samples require no treatment when shipped with ice packs at ambient temperature.

4)When shipping with dry ice, samples should be cut into small pieces, each less than 1 cm³.

 

5.Packing and Shipping

 

1)Store samples in clearly labeled tubes, label with sample name, concentration, and preparation date. Ensure tube tops are sealed.

2)For transport, secure sample tubes in 50ml capped centrifuge tubes placed in sealable bags. Use dry ice or ice packs for transportation, avoiding repeated freeze-thaw cycles. DO NOT send the samples out on Fridays, to avoid weekend delays.

 

6.Labeling Requirements

 

1)Employ double labeling, ensuring clear identification on both the sample tubes and external packaging.

2)To prevent confusion, avoid simplistic labels like A/B/C or 1/2/3. Instead, use a combination of letters and numbers for unique sample identification, reducing the risk of duplication.

 

7.Additional Information

 

A minimum of 24 analyses is required. If fewer than 24 analyses are conducted, the cost will be calculated as if 24 analyses were performed.