Amylose Content Assay

Starch granules are large particles assembled from amylose and amylopectin molecules, with the content of amylose always being a critical indicator for evaluating the quality of starch.The main methods for determining the content of amylose are: Gel Permeation Chromatography(GPC) and colorimetry. The GPC method is relatively complex and costly in operation, but it can provide multiple important parameters such as the molecular weight distribution of starch. The colorimetric method mainly uses the different affinity of amylose and amylopectin to iodine, and prepares standard curves by preparing different proportions of amylose/branched chain standard solution, so as to quantitatively determine the amylose content in the sample.

 

Workflow

 

Why Choose GlycoSpectra Analytics

1.High-throughput, capable of analyzing 96 samples at once.

 

2.Both whole powder and starch samples can be tested.

 

3.Convenient and fast, results available within 20 working days.

 

4.Professional and thorough service, offering pre-sale consultations and post-sale technical support.

 

Sample Submission Guidelines

1.Sample Types

 

Starch or raw samples are both acceptable.

 

2.Biological Replicates

 

A minimum of three biological replicates is recommended for robust results.

 

3.Quantity Requirements

 

Raw sample >2 g; Starch >1 g.

 

4.Sample Preparation

 

1)Establish both control and experimental groups, ensuring a minimum of three biological replicates.

2)Ensure maintain consistency in timing whenever possible.

3)Minimize preprocessing; we prefer to handle these steps. Clients should ensure liquid samples are securely packaged in non-glass containers and buffered against shock and impact. Dry solid samples can be untreated or preliminarily ground. Fresh solid samples require no treatment when shipped with ice packs at ambient temperature.

4)When shipping with dry ice, samples should be cut into small pieces, each less than 1 cm³.

 

5.Packing and Shipping

 

1)Store samples in clearly labeled tubes, label with sample name, concentration, and preparation date. Ensure tube tops are sealed.

2)For transport, secure sample tubes in 50ml capped centrifuge tubes placed in sealable bags. Use dry ice or ice packs for transportation, avoiding repeated freeze-thaw cycles. DO NOT send the samples out on Fridays, to avoid weekend delays.

 

6.Labeling Requirements

 

1)Employ double labeling, ensuring clear identification on both the sample tubes and external packaging.

2)To prevent confusion, avoid simplistic labels like A/B/C or 1/2/3. Instead, use a combination of letters and numbers for unique sample identification, reducing the risk of duplication.