Nuclear Magnetic Resonance Spectroscopy (NMR) Based Polysaccharide Analysis Service

The principle of Nuclear Magnetic Resonance (NMR) primarily involves the utilization of atomic nuclei undergoing energy level splitting within a strong magnetic field. This process leads to transitions in nuclear spin energy levels upon the absorption of external electromagnetic radiation, thus generating nuclear magnetic resonance. NMR analysis is instrumental in determining the anomeric configuration, types of glycosidic linkages, and sequencing of polysaccharides by capturing proton chemical shifts in a high-frequency magnetic field. The cornerstone spectra in 1D NMR encompass 1H and 13C NMR, applied to delineate chemical shifts of carbon and hydrogen in sugar residues. Owing to the considerable signal overlap of identical atoms in 1D NMR, advanced 2D NMR techniques, including COSY, HSQC, and HMBC, are employed to elucidate the molecular structure of polysaccharides.

 

GlycoSpectra Analytics leverages high-resolution NMR spectrometers designed for exceptional data processing speeds and substantial storage capabilities across one-dimensional, two-dimensional, and multidimensional spectra. Our spectrometers, featuring high sensitivity and gradient fields, are exceptionally suited for analyzing high molecular weight polymers. Furthermore, we pride ourselves on a distinguished scientific and technical team dedicated to providing professional data interpretation services.

 

Workflow

 

Why Choose GlycoSpectra Analytics

1.Our facility is equipped with cutting-edge, high-resolution NMR spectrometers, optimized for comprehensive data processing and storage for extensive spectral analysis.

 

2.The advanced system requires minimal sample volumes while offering unparalleled sensitivity, making it ideal for the scrutiny of high molecular weight polymers.

 

3.Our adept team offers expert spectroscopic interpretation services, backed by extensive experience.

 

Sample Submission Guidelines

1.Sample Types

 

Purified polysaccharide freeze-dried powder.

 

2.Quantity Requirements

 

50~100 mg.

 

3.Sample Preparation

 

1)Polysaccharides/oligosaccharides after column purification and desalting by dialysis or ultrafiltration.

2)The absolute purity of polysaccharides is higher than 60%.

3)It is best to have methylation data for reference.

 

4.Packing and Shipping

 

Please place the sample in a suitable container and indicate necessary information such as sample name, concentration, and preparation time.

 

5.Other

 

A sample technical requirement form must be mailed along with the samples to avoid delays in project timelines due to the inability to promptly verify information.