Polysaccharide Methylation Analysis

The chemical structure of polysaccharide is the basis of its biological activity, and changes in structure will affect its biological activity. Because it is a complex biological macromolecule, the complexity of its structure brings great difficulties to the polysaccharide elucidation process. There are many methods for the structural analysis of polysaccharides, including infrared, nuclear magnetic resonance, mass spectrometry and other instrumental analysis methods, as well as chemical methods such as acid hydrolysis and methylation reactions, as well as biological methods such as glycosidase digestion and immunological analysis.

 

Methylation analysis is one of the most powerful tools for structural analysis of polysaccharides and oligosaccharides. Partially methylated sugar alcohol acetyl derivatives can be obtained for qualitative and quantitative analysis by combined gas chromatography-mass spectrometry (GC-MS) to determine the ligation of the individual monosaccharide residues.

 

Workflow

 

Why Choose GlycoSpectra Analytics

1.Good reproducibility and high data accuracy.

 

2.The detection index is complete, and acidic, neutral and modified polysaccharides can be determined.

 

3.Rich experience and completion of numerous projects.

 

4.Thoughtful service, provide pre-sales consultation, after-sales technical support.

 

Sample Submission Guidelines

1.Sample Types

 

Purified polysaccharide freeze-dried powder.

 

2.Quantity Requirements

 

≥20mg.

 

3.Sample Preparation

 

1)Polysaccharides/oligosaccharides after column purification and desalting by dialysis or ultrafiltration.

2)The absolute purity of polysaccharides is higher than 20%.

3)There are monosaccharide composition and gel chromatogram reference.

 

4.Packing and Shipping

 

Please place the sample in a suitable container and indicate necessary information such as sample name, concentration, and preparation time.

 

5.Other

 

A sample technical requirement form must be mailed along with the samples to avoid delays in project timelines due to the inability to promptly verify information.