Polysaccharide &Monosaccharide Composition Analysis

The chemical structure of polysaccharides is the basis of their biological activity, and changes in structure can affect their bioactivity. As a structurally complex biomolecule, the structural characteristics of polysaccharides pose significant challenges to the analysis of their functions. There are three common methods for determining monosaccharide composition: High Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), and High Performance Anion Exchange Chromatography-Pulsed Amperometric Detector (HPAEC-PAD). Due to the complex operations, low precision, and long cycles of HPLC and GC methods, which often require pre-column derivatization of monosaccharides leading to the formation of isomers and large errors, the HPAEC-PAD method has become the mainstream method for monosaccharide composition analysis due to its advantages of high sensitivity, efficiency, simplicity of operation, and good repeatability. GlycoSpectra Analytics utilizes HPAEC-PAD technology to achieve rapid qualitative and quantitative analysis of monosaccharides, and can analyze nearly all monosaccharides and most oligosaccharides and polysaccharides.

 

Workflow

 

List of Analysis Service

 

Why Choose GlycoSpectra Analytics

1.Advanced equipment, Thermo ion chromatography system equipped with a chromatography column specifically designed for carbohydrate chemistry.

 

2.High sensitivity, with a lower limit of detection as low as 0.5μg/mL.

 

3.Good repeatability, no need for derivatization, reducing experimental errors and ensuring high data accuracy.

 

4.Convenient and fast, enabling rapid qualitative and quantitative analysis of various monosaccharides and oligosaccharides, with the option for custom indicators.

 

Sample Submission Guidelines

1.Sample Types

 

Polysaccharide freeze-dried powder.

 

2.Biological Replicates

 

A minimum of three biological replicates is recommended for robust results.

 

3.Quantity Requirements

 

Solid (dry sample): ≥10mg.

 

4.Sample Preparation

 

1)Liquid samples (if they contain salt, it is recommended to desalt them first) need to be freeze-dried or vacuum-dried first to ensure that they are fully dried and do not contain moisture and organic solvents.

2)In order to accurately reflect the sample results, the sampling needs to be uniform.

3)It is recommended to purify the polysaccharide and send it to our company after collecting the materials.

 

5.Packing and Shipping

 

Please place the sample in a suitable container and indicate necessary information such as sample name, concentration, and preparation time.

 

6.Labeling Requirements

 

1)Employ double labeling, ensuring clear identification on both the sample tubes and external packaging.

2)To prevent confusion, avoid simplistic labels like A/B/C or 1/2/3. Instead, use a combination of letters and numbers for unique sample identification, reducing the risk of duplication.

 

7.Other

 

A sample technical requirement form must be mailed along with the samples to avoid delays in project timelines due to the inability to promptly verify information.