Starch Chain Length Distribution Analysis Service
Starch is categorized into two types according to the glucose unit bonding pattern: amylose and amylopectin. Amylose primarily comprises glucose units linearly linked through α-1,4-glycosidic bonds. In contrast, amylopectin possesses a branched structure, formed by both α-1,4 and α-1,6-glycosidic bonds. Starch chain length distribution analysis involves the examination of glucan chains of various lengths that are liberated by hydrolyzing starch’s 1,6-glycosidic bonds using isoamylase. Subsequently, this process entails the quantification of glucan chains exhibiting varying polymerization degrees via Ion Chromatography (IC) or Gel Permeation Chromatography (GPC).
GlycoSpectra Analytics utilizes two sophisticated methodologies to determine starch chain length distributions: High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD, also referred to as IC) and Gel Permeation Chromatography (GPC, also known as Size Exclusion Chromatography, SEC). HPAEC-PAD is tailored specifically for the quantification of amylopectin branch lengths within the DP6-DP76 range, rendering it unsuitable for linear starch chain analysis. GPC covers a wider range of measurements, from several dozen to several thousand, suitable for both branched and straight-chain starches. However, GPC exhibits limited precision for shorter chains (degree of polymerization under 10).
Workflow
Why Choose GlycoSpectra Analytics
1.We offer client-centric approaches, incorporating efficient anion exchange chromatography and gel permeation chromatography.
2.Our integration of gel permeation chromatography with a DMSO system for measurement stands as a domestic forefront.
3.We provide professional, comprehensive services, including hands-on assistance with post-sale data analysis.
4.Our team has rich experience and has completed a lot of projects.
Sample Submission Guidelines
1.Sample Types
Starch samples, clients can provide the original sample for starch extraction by our company, which is subject to a separate charge.
2.Biological Replicates
A minimum of three biological replicates is recommended for robust results.
3.Quantity Requirements
Raw sample > 50 g; Starch >1 g.
4.Sample Preparation
1)Establish both control and experimental groups, ensuring a minimum of three biological replicates.
2)Ensure maintain consistency in timing whenever possible.
3)Minimize preprocessing; we prefer to handle these steps. Clients should ensure liquid samples are securely packaged in non-glass containers and buffered against shock and impact. Dry solid samples can be untreated or preliminarily ground. Fresh solid samples require no treatment when shipped with ice packs at ambient temperature.
4)When shipping with dry ice, samples should be cut into small pieces, each less than 1 cm³.
5.Packing and Shipping
1)Store samples in clearly labeled tubes, label with sample name, concentration, and preparation date. Ensure tube tops are sealed.
2)For transport, secure sample tubes in 50ml capped centrifuge tubes placed in sealable bags. Use dry ice or ice packs for transportation, avoiding repeated freeze-thaw cycles. DO NOT send the samples out on Fridays, to avoid weekend delays.
6.Labeling Requirements
1)Employ double labeling, ensuring clear identification on both the sample tubes and external packaging.
2)To prevent confusion, avoid simplistic labels like A/B/C or 1/2/3. Instead, use a combination of letters and numbers for unique sample identification, reducing the risk of duplication.
7.Additional Information
A minimum of 24 analyses is required. If fewer than 24 analyses are conducted, the cost will be calculated as if 24 analyses were performed.