Total Starch Content Determination Service

Starch serves as a critical nutrient reservoir in plant structures, primarily in seeds and tubers. It is a prevalent component across diverse plant species, characterized by varying concentrations based on the specific type of plant material. Conventional methods for assessing total starch content, such as the dual-wavelength or sulfuric acid techniques, often exhibit limited stability and precision. Furthermore, the presence of substantial soluble sugars in biological samples can impede accurate starch quantification. GlycoSpectra Analytic circumvents these issues by initially extracting soluble sugars from the samples. The subsequent steps of enzymatic hydrolysis followed by colorimetric analysis facilitate precise starch content determination, ensuring results with enhanced accuracy and consistency.

 

Workflow

 

Why Choose GlycoSpectra Analytic

1.Our Enzymatic hydrolysis method, combined with the Glucose Oxidase- Peroxidase(GOPOD) colorimetric assay, provides excellent stability, exhibiting a Relative Standard Deviation (RSD) of less than 5% within the same batch.

 

2.This technique effectively mitigates the influence of soluble sugars, yielding more dependable results.

 

3.Our process is streamlined and efficient, delivering results within 20 working days.

 

4.We offer a full spectrum of services, encompassing pre-sale consultations and post-sale technical support.

 

Sample Submission Guidelines

1.Sample Types

 

Fresh or dry samples are both acceptable.

 

2.Biological Replicates

 

A minimum of three biological replicates is recommended for robust results.

 

3.Quantity Requirements

 

Fresh Sample > 2 g; Dry Sample >1 g;

 

4.Sample Preparation

 

1)Establish both control and experimental groups, ensuring a minimum of three biological replicates.

2)Ensure maintain consistency in timing whenever possible.

3)Minimize preprocessing; we prefer to handle these steps. Clients should ensure liquid samples are securely packaged in non-glass containers and buffered against shock and impact. Dry solid samples can be untreated or preliminarily ground. Fresh solid samples require no treatment when shipped with ice packs at ambient temperature.

4)When shipping with dry ice, samples should be cut into small pieces, each less than 1 cm³.

 

5.Packing and Shipping

 

1)Store samples in clearly labeled tubes, label with sample name, concentration, and preparation date. Ensure tube tops are sealed.

2)For transport, secure sample tubes in 50ml capped centrifuge tubes placed in sealable bags. Use dry ice or ice packs for transportation, avoiding repeated freeze-thaw cycles. DO NOT send the samples out on Fridays, to avoid weekend delays.

 

6.Labeling Requirements

 

1)Employ double labeling, ensuring clear identification on both the sample tubes and external packaging.

2)To prevent confusion, avoid simplistic labels like A/B/C or 1/2/3. Instead, use a combination of letters and numbers for unique sample identification, reducing the risk of duplication.

 

7.Additional Information

 

A minimum of 24 analyses is required. If fewer than 24 analyses are conducted, the cost will be calculated as if 24 analyses were performed.